日粮添加绿原酸和橙皮苷对断奶仔猪生长性能与肠道功能的影响

doi:10.11843/j.issn.0366-6964.2019.03.012

摘要/Abstract

摘要：

旨在研究日粮添加绿原酸（chlorogenic acid，CHA）和橙皮苷（hesperidin，HDN）对断奶仔猪生长性能与肠道功能的影响。本研究选择40头健康长白×大白×荣昌猪三元杂交断奶阉公仔猪（（28±2）d，（8.97±1.48）kg），按单因素随机分组分为4组：基础日粮组（CON）、杆菌肽组（BT）、绿原酸组（CHA）、橙皮苷组（HDN）。每组10个重复，每个重复1头，采用单栏饲养。试验期共33 d，其中预试期5 d。测定生长性能、血浆和肝生化指标、肠道黏膜组织形态、肠道黏膜相关蛋白含量与mRNA丰度、肠道微生物区系。结果表明：1）与对照组相比，日粮中添加CHA和HDN能显著提高断奶仔猪平均日增重、平均日采食量、胸腺指数和仔猪小肠长度指数（P<0.05）；显著提高血浆和肝组织中总抗氧化能力、总超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶、谷胱甘肽过氧化物酶活力（P<0.05）。2）CHA、HDN和BT组仔猪空肠绒毛高度和完整性上均优于对照组（P<0.05）。3）CHA组仔猪β-defensins 2蛋白水平显著高于对照组（P<0.05），CHA组仔猪空肠黏膜TNF-α mRNA丰度显著高于HDN组和对照组（P<0.05），HDN组仔猪空肠黏膜IL-8 mRNA丰度高于BT组（P<0.05）。仔猪空肠黏膜HSP₉₀和TLR4蛋白质水平以及TGF-β1 mRNA丰度各处理组间差异不显著（P>0.05）。4）CHA和HDN能维持断奶仔猪结肠微生物区系的多样性，保护肠道微生态平衡。研究结果显示，日粮添加绿原酸和橙皮苷能显著提高断奶仔猪的平均日增重、平均日采食量、空肠黏膜绒毛高度，显著降低料肉比。绿原酸通过促进肠道上皮BD2的表达发挥其抗菌作用，绿原酸通过提高TNF-α发挥抗炎作用，绿原酸和橙皮苷具有抗氧化作用，能维持断奶仔猪肠道微生物区系的多样性。

Abstract:

The objective of this study was to investigate the effects of dietary chlorogenic acid (CHA) and hesperidin (HDN) on growth performance and intestinal function in weaned piglets. Forty Landrace×Yorkshire×Rongchang healthy castrated weaned male piglets ((28±2) d, (8.97±1.48) kg) were randomly divided into 4 groups (10 repeat per group, 1 piglet per repeat):control group (CON), Bacilli peptide group(BT), HDN group, CHA group. The experiment lasted for 33 d including 5 d adaptation period. The growth performance, biochemical indicators of plasma and liver, intestinal mucosa morphology, the expression of genes and proteins related to intestinal mucosa and colon microflora were determined. The results showed that:1) Compared with control group, the addition of CHA and HDN in basal diet of weaned piglets significantly increased the average daily gain, average daily feed intake, thymus index and small intestine length index (P<0.05), and significantly improved the total antioxidant capacity and the activity of total superoxide dismutase, catalase, glutathione reductase, glutathione peroxidase (P<0.05). 2) The height and integrity of jejunum villus of piglets in CHA, HDN and BT groups were better than those in CON group (P<0.05). 3) The β-defensins 2 concentration of piglets in CHA group were higher than those in CON group (P<0.05). The TNF-α mRNA level in jejunum villus of piglets in CHA group was higher than those in HDN and CON groups (P<0.05). The IL-8 mRNA level in jejunum villus of piglets in HDN group was higher than those in BT group (P<0.05). There was no significant difference in HSP₉₀ and TLR4 protein levels and TGF-β1 mRNA abundance among 4 groups (P>0.05). 4) CHA and HDN could maintain the diversity of the colonic microbiological flora of weaned piglets and protect the intestinal micro-ecological balance. The result indicate that dietary supplementation of chlorogenic acid and hesperidin increased average daily gain, average daily feed intake, jejunal mucosal villus height, and significantly reduced the ratio of feed to meat of weaned piglets. Chlorogenic acid plays an anti-bacterial role by promoting the expression of BD2 in intestinal epithelium and plays an anti-inflammatory role by enhancing TNF-α. Chlorogenic acid and hesperidin can maintain the diversity of intestinal microflora in weaned piglets.

中图分类号:

S816.79
S828

赖星, 陈庆菊, 卢昌文, 马娅君, 唐志如. 日粮添加绿原酸和橙皮苷对断奶仔猪生长性能与肠道功能的影响[J]. 畜牧兽医学报, 2019, 50(3): 570-580.

LAI Xing, CHEN Qingju, LU Changwen, MA Yajun, TANG Zhiru. The Effects of Dietary Chlorogenic Acid and Hesperidin on Growth Performance and Intestinal Function in Weaned Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 570-580.

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